Spectrophotometry is a special technique used for measuring the quantity of light that a chemical substance absorbs. It uses a quantitative measurement. This can be done via the measurement of light intensity passing through a given sample regarding the light intensity via a blank or reference sample. The technique has so many applications and can be used for many types of samples. Some of the samples include the following:
- Glass
- Thin-film
- Solids
- Liquids
The spectrophotometer can be used for series of analytical applications and it is adopted by many industries because of its accuracy. Its precision measurement cannot be compared to anything else. The wavelength generated by the product is between 190nm and 1,100nm. You will always get value for money when you use UV-Vis Spectroscopy.
Check below for more of the benefits of using the tool.
Outstanding benefits
Spectrophotometer is reliable for determining the concentration or amount of a particular chemical substance by simply paying calculating the number of photons reaching the detector. The concentration of the particular substance is very high if it absorbs a lot of light from a given wavelength. UV-Vis Spectroscopy is being used in several applications in the since world and some of these applications will be discussed next to give you a better understanding of how the tool works.
Spectrophotometer applications
Spectrophotometer is applicable in traditional chemistry thanks to its incomparable accuracy and precision measurement. It has also found use in the medical profession. In fact, it is used extensively in ophthalmology with very good results. It has equally found use in microbiology where the critical studies of microorganisms and their activities are in focus. Aside from the above, some other areas where this tool has found use are highlighted below:
- Quality control
- Cosmetic industry
- Petrochemistry
- Pharmaceutical research
- Optical components
- Material sciences
- Food & agriculture
The tool can measure light absorption as we have mentioned earlier. It measures light as they are reflected, transmitted and absorbed by a given sample in a particular wavelength range. The wavelength range of the product is 800 to 3200 nm.
Popular uses
Spectrophotometer can be used for series of purposes and we are going to highlight them below:
- It can be used for confirming the identity-comparison of a given spectrum while referencing
- You can also use it for measuring concentration
- It has found an extensive use in reaction kinetics also, as well as, color measurement.
- If you need the perfect tool for the structural changes of compounds, this tool will work perfectly for that. It can also function in proteins conformational studies.
- The tool is reliable for multi-component analysis.
- It can equally be used for multi-temperature analysis.
Its effectiveness is one of the several features that stand the tool out.
